CHEM07004 2019 SEPARATION SCIENCE FOR FORENSICS
This module covers a range of analytical separation techniques. It address the fundamental science involved in separation science and includes instrumental and non instrumental separation methodologies. The module also addresses sample preparation and pre treatments with particular emphasis on trace organic analysis in forensically relevant samples.
On completion of this module the learner will/should be able to;
Explain the separation process in column chromatography.
Describe the chemistries of some of the phases used in instrumental chromatographic techniques.
Perform and interpret both qualitative and quantitative analytical chromatographic separations.
Evaluate the limitations, accuracies and precision of various separation techniques.
Communicate scientific data in an appropriate format.
Teaching and Learning Strategies
This module will be taught through lecture, practical workshop and tutorial. Students will learn through active engagement with each of these learning environments. Students will also be directed to online learning resources appropriate to this module.
Module Assessment Strategies
Assessment will be through practical assessment, short questions and final examination.
Repeat continuous assessment and / or final examination
Intermolecular interactions and partition coefficients.
Sample preparation techniques: Solvent and solid phase extraction techniques, derivatisation as applied for forensic samples.
Theories of band broadening in column chromatography and definition of terms (efficiency, capacity factor, selectivity, resolution, van Deemter)
Overview of stationary and mobile phases in column chromatography (Elutotropic series of solvents and column stationary phase chemistries).
Practice of instrumental separations, e.g. HPLC, GC, IC, GCMS.
Validation of chromatographic methods.
Qualitative and quantitative evaluation and interpretation of chromatographic separations.
Coursework & Assessment Breakdown
|Title||Type||Form||Percent||Week||Learning Outcomes Assessed|
|1||Short Questions.||Formative||UNKNOWN||- %||OnGoing||1,2,3|
|2||Short Questions.||Coursework Assessment||Closed Book Exam||10 %||OnGoing||1,2,3|
|3||Laboratory Skills and Report Writing.||Coursework Assessment||Closed Book Exam||40 %||OnGoing||4,5|
End of Semester / Year Assessment
|Title||Type||Form||Percent||Week||Learning Outcomes Assessed|
|1||Final Examination.||Final Exam||Closed Book Exam||50 %||End of Semester||1,2,3,4,5|
Full Time Mode Workload
|Practical / Laboratory||Science Laboratory||Chemistry Laboratory||2||Weekly||2.00|
|Independent Learning||UNKNOWN||Self Study||3||Weekly||3.00|
Required & Recommended Book List
2010-01-15 Forensic Applications of High Performance Liquid Chromatography CRC Press
ISBN 1420091913 ISBN-13 9781420091915
Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective. Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines: The history of HPLC and the theory behind the separation process The requirements for successful analysis and best practice tips The modes of separation and detection most appropriate for forensic science applications HPLC method development and evaluation The quality aspects of laboratory operation Troubleshooting HPLC systems and analyses Applications of HPLC within the field of forensic science Designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers. An instructors manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption
1992-10-31 Gas Chromatography In Forensic Science Intellect Books
ISBN 0133271986 ISBN-13 9780133271980
Describes the application of gas chromatography to various aspects of forensic chemistry. Following an introduction to the basic theory of chromatographic separations, the text discusses specific issues, such as drug analysis, fires and explosives, alcohol and toxicology.
2015-05-29 Quantitative Chemical Analysis WH Freeman
ISBN 131915414X ISBN-13 9781319154141
The gold standard in analytical chemistry, Dan Harris Quantitative Chemical Analysis provides a sound physical understanding of the principles of analytical chemistry and their applications in the disciplines. Dan Harris presents the subject in a rigorous, readable and interesting manner, lucid enough for non-chemistry majors but containing the depth required by advanced undergraduates. For the first time since the book was published in 1978, Harris has worked with a new contributing author, Professor Chuck Lucy of the University of Alberta. Lucy shares his expertise and teaching experience in Chapters 23-26 on chromatography and capillary electrophoresis.
Quantitative Chemical Analysis, Daniel C. Harris, 9th Edition, 2015.
Practical Laboratory Skills Training Guide: HPLC, E. Prichard, B Stuart, RSC, 1st Ed., 2003.
Chromatography: Concepts and Contrast, 2ed. James Miller, Wiley, 2009.
Chromatography and Separation Science, Satinder Ahaja, Academic Press, 1st Ed. 2002.
Basic Gas Chromatography, 2ed., Harold M. Miller, James M. Miller, Willey-Interscience, 2009.
Gas Chromatography, 2ed., Ian A. Fowlis, Wiley India Pvt Ltd, 2008.
Practical High Performance Liquid Chromatography, Veronica R. Meyer, John Wiley & Sons, 2010.
Handbook of Forensic Drug Analysis, Fred Smith, Jay A. Siegel, Academic Press, 2004.
Introduction to Modern Liquid Chromatography, 3rd Ed. Lloyd R. Snyder, Joseph J. Kirkland, John W. Dolan, Wiley, 2009.
The Analysis of Controlled Substances, Michael D. Cole, J. Wiley & Sons, 2003.
Fundamentals of Analytical Toxicology, Robert J Flanagan, Andrew Taylor, Ian D. Watson, Robin Whelpton, Wiley, 2007.
Forensic Applications of High Performance Liquid Chromatography, Shirley Bayne, Michelle Carlin, CRC Press, 2010.
Gas Chromatography in Forensic Science, Ed. Ian Tebbett, CRC Press, 1992.
Forensic Applications of Gas Chromatography, Michelle Groves carlin, John Richard Dean, CRC Press, 2013.