BIOC09004 2023 Cell Processing 1
Full Title
Cell Processing 1
Transcript Title
Cell Processing 1
Code
BIOC09004
Attendance
N/A %
Subject Area
BIOC - 0512 Biochemistry
Department
LIFE - Life Sciences
Level
09 - Level 9
Credit
05 - 05 Credits
Duration
Semester
Fee
€
Start Term
2023 - Full Academic Year 2023-24
End Term
9999 - The End of Time
Author(s)
Rachel Coyle, Ailish Breen
Programme Membership
SG_SATMQ_S09 202300 Postgraduate Certificate in Advanced Therapy Medicinal Products (ATMP) Cell Manufacturing
SG_SATMR_M09 202300 Master of Science in Advanced Therapy Medicinal Products (ATMP) Cell Manufacturing
SG_SATMP_M09 202300 Master of Science in Advanced Therapy Medicinal Products (ATMP) Technology and Manufacturing
SG_SATMP_O09 202300 Postgraduate Diploma in Science in Advanced Therapy Medicinal Products (ATMP) Technology and Manufacturing
Description
The Cell Processing 1 module introduces the basic concepts and issues pertaining to the culture of mammalian cells. The module outlines the growth requirements of cells in culture including the various media which may be used and how cells are grown and stored by cryopreservation. Issues relating to chemical and biological contaminations which may impact on cell culture are described. Identification of cell viability using visual techniques and assays are discussed. Regulatory requirements for starting materials in cell culture, cell identification and cell purity are also discussed.
Learning Outcomes
On completion of this module the learner will/should be able to;
1.
Describe the basics of mammalian cell culture, cell growth and cryopreservation, as it applies to producer cell lines and packaging cell lines
2.
Explain the different types and formulations of media / starting materials available and examine their overall importance in optimising the cell culture process and ensuring integrity of the culture
3.
Evaluate contamination and biosafety issues and propose in process QC analytical test methods
4.
Appraise different cell identification markers for their role in QC analytics
5.
Problem solve simulated cell processing quality issues through case studies
Teaching and Learning Strategies
This Module will be delivered online part time.
A virtual learning environment (e.g. Moodle) will be used as a repository of educational resources and as a means of assessment. Self directed, student centred, independent learning is a core aspect through completion of module coursework. Some aspects of flipped learning, groupwork, case studies and other active learning methodologies will be incorporated into the module.
Module Assessment Strategies
The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQ's, project assignments, essays, oral presentations, vivas etc. The major piece of assessment will incorporate a case study or simulated data, and requires students to propose solutions and defend their decisions in an oral presentation.
Repeat Assessments
Repeat continuous assessment of the failed elements in line with marks and standards
Indicative Syllabus
1. Describe the basics of mammalian cell culture, cell growth and cryopreservation, as it applies to producer cell lines and packaging cell lines
- Cell culture principles
- Cell lines (adherent and suspension) and their applications with a focus on ATMP relevant cells (e.g. HeLa, Vero, 393, different stem cells, T cells etc.) , thawing, cell cycle, cryopreservation
2. Explain the different types and formulations of media / starting materials available and examine their overall importance in optimising the cell culture process and ensuring integrity of the culture
- Media composition
- Media and additives, use of serums, antibiotics and their implications
- Sourcing cells and starting materials
3. Evaluate contamination and biosafety issues and propose in process QC analytical test methods
- Identification of contaminants
- Types of contaminants and QC tests
- Aseptic technique
4. Appraise different cell identification markers for their role in QC analytics
- Cell identification and viability
- Morphology, cell surface markers, cell counting, cell viability testing assays, flow cytometry
5. Problem solve simulated cell processing quality issues through case studies
- Case studies of biocontamination, issues with cell growth and starting materials etc.
Coursework & Assessment Breakdown
Coursework & Continuous Assessment
100 %
Coursework Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | Quiz | Coursework Assessment | Multiple Choice/Short Answer Test | 15 % | Week 7 | 1,2 |
| 2 | Short answer quiz | Coursework Assessment | Assessment | 15 % | Week 10 | 3,4 |
| 3 | Case study | Coursework Assessment | Assignment | 55 % | End of Semester | 2,3,4,5 |
| 4 | Presentation | Coursework Assessment | Oral Exam/Presentation | 15 % | End of Semester | 2,3,4,5 |
Online Learning Mode Workload
| Type | Location | Description | Hours | Frequency | Avg Workload |
|---|---|---|---|---|---|
| Lecture | Online | Lecture | 2 | Weekly | 2.00 |
| Independent Learning | Not Specified | Directed and self directed learning | 4 | Weekly | 4.00 |
Total Online Learning Average Weekly Learner Contact Time 2.00 Hours
Required & Recommended Book List
Recommended Reading
2013-12-12 Stem Cell Bioprocessing Woodhead Publishing
ISBN 1907568883 ISBN-13 9781907568886
2013-12-12 Stem Cell Bioprocessing Woodhead Publishing
ISBN 1907568883 ISBN-13 9781907568886
Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine.
Recommended Reading
Bioprocessing of Viral Vaccines CRC Press
Bioprocessing of Viral Vaccines CRC Press
Recommended Reading
2021-03-17 Freshney's Culture of Animal Cells John Wiley & Sons
ISBN 9781119513049 ISBN-13 1119513049
2021-03-17 Freshney's Culture of Animal Cells John Wiley & Sons
ISBN 9781119513049 ISBN-13 1119513049
FRESHNEYS CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshneys Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshneys Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.
Module Resources
Non ISBN Literary Resources
Updated Literary Resources
Journal Resources
Kimura, T., Ferran, B., Tsukahara, Y. et al. Production of adeno-associated virus vectors for in vitro and in vivo applications. Sci Rep 9, 13601 (2019). https://doi.org/10.1038/s41598-019-49624-w
Other journal articles as appropriate
URL Resources
Other Resources
Cell Therapy Handbook. by Thermofisher Scientific. Available at: https://www.thermofisher.com/ie/en/home/global/forms/cell-therapy-handbook.html
Additional Information