BIO09046 2013 Upstream Bioprocessing Practical

Demonstrate a range of practical know-how and skills associated with relevant, lab-based upstream bioprocessing equipment for the Biopharmaceutical Processing industry.

Demonstrate a range of practical know-how and skills associated with relevant, pilot-plant based upstream bioprocessing equipment for the Biopharmaceutical Processing industry.

Interpret and critically evaluate the data and results obtained from the various upstream processing practicals.

Take responsibility for and direct the work of other individuals in a GMP- like environment.

Demonstrate research skill and capabilities in the overall upstream bioprocessing area.

Evaluate ongoing education and training needs in a continuous professional development environment.

Demonstrate effective communication skills both orally and in writing.

As this is a practical-based module attendance at the NIBRT or IT Sligo training laboratories is a requirement. A certain amount of the assessment work will take place at these locations. The remaining assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQ’s/SAQ’s, project assignments/reports, practical/project work, essays, oral presentations, vivas etc.

The range of lab-based practicals covered may include some or all of the following items: aseptic techniques; cell culture techniques including cell counting (both manual and automated); sub-culturing including media exchange; thawing up and freezing-down of cells; benchtop bioreactor techniques such as disassembly/reassembly of parts; bioreactor instrument calibration; preparation of bioreactor for autoclaving; inoculation and operation of bioreactor; sampling and substrate analysis.

The range of pilot-plant based practicals covered may include some or all of the following items: P+ID (Piping and Instrumentation Diagrams) analysis work on bioreactor systems; CIP cycle operation on bioreactor systems; SIP cycle operation on bioreactor systems; cell culture run on the bioreactor vessel; operation of the perfusion skid system for the bioreactor vessel; calibration of pH and DO instruments; kla measurement on the bioreactor vessel under different operating conditions; aseptic sampling from the harvest vessel using the Novaseptum system; operation of the disc-stack centrifuge separating system; P+ID analysis work on the microfiltration skid system; operation of the microfiltration system; operation of the depth filtration skid system.