BIO08045 2019 Cell Culture Processing
This module aims to provide learners with a deep understanding of the theory of mammalian cell culture processing as it pertains to the biosynthesis of modern biopharmaceutical products. It will examine the processing flow from cell vial thaw and subsequent scale-up through to protein synthesis and product formation. Consideration will be given to the development and characterisation of cell lines for use in biomanufacturing, the kinetics of cell growth and media requirements to optimise cell performance and productivity. The learner will be exposed to a detailed comparative discussion of mammalian cell bioreactor design, operation, and control for the successful manufacture of biotherapeutic proteins.
Learning Outcomes
On completion of this module the learner will/should be able to;
Describe the fundamentals of mammalian cell biology and cell line development and explain the process kinetics relating to cell growth and productivity for a bioreactor process.
Identify the key components of mammalian cell culture media formulations and relate those to the optimisation of a cell culture process.
Compare and contrast the most common bioreactor designs available, their key features¸ and the relative advantages and disadvantages in different operating modes.
Describe the key steps in bioreactor operation, the critical control parameters and the main operating and control loops associated with bioreactor performance monitoring.
Analyse, evaluate and draw conclusions from data obtained from lab. demonstrations and/or data obtained from problem solving exercises.
Perform research on a relevant topic and communicate in writing and orally an informed and evidence based analysis and evaluation of current thinking, with appropriate conclusions.
Teaching and Learning Strategies
as above
Module Assessment Strategies
Assessment Strategies
Continuous Assessment of Course Work - 30% of marks.
End of term online exam - 30% of marks.
Mini research project - 40% of marks.
The miniproject is a MANDATORY form of assessment for any modules on this programme with a miniproject component.
Repeat Assessments
as per Q Manual
Module Dependencies
Indicative Syllabus
Indicative Syllabus
- Overview of cell biology relating to cell culture processing and bioreactor operations.
- Kinetics of cell growth, yield and productivities, cell counting techniques, cell density and cell viability measurements.
- Cell culture media design and formulation.
- Energy and mass transfer systems for bioprocessing.
- Bioreactor design and engineering.
- Bioreactor operation and control of critical parameters for protein synthesis.
Coursework & Assessment Breakdown
Coursework Assessment
Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
---|---|---|---|---|---|---|
1 | Open Book Exam Online | Coursework Assessment | UNKNOWN | 15 % | Week 4 | 1,2 |
2 | Open Book Exam Online. | Coursework Assessment | UNKNOWN | 15 % | Week 9 | 3,4 |
3 | Other Exam Online. | Coursework Assessment | Closed Book Exam | 30 % | Week 14 | 1,2,3,4,5 |
4 | Individual Project Research and Problem Solving | Coursework Assessment | UNKNOWN | 40 % | Week 15 | 6 |
Part Time Mode Workload
Type | Location | Description | Hours | Frequency | Avg Workload |
---|---|---|---|---|---|
Lecture | Not Specified | Online lecture | 2 | Weekly | 2.00 |
Directed Learning | Not Specified | Course Notes. | 4 | Weekly | 4.00 |
Independent Learning | UNKNOWN | Mini Research Activities. | 4 | Weekly | 4.00 |
Required & Recommended Book List
2007-08-13 Pharmaceutical Biotechnology John Wiley & Sons
ISBN 9780470012444 ISBN-13 0470012447
Offers a comprehensive introduction to the principles and concepts of pharmaceutical biotechnology.
2001-04-19 Basic Biotechnology Cambridge University Press
ISBN 0521779170 ISBN-13 9780521779173
Biotechnology: what it is, how it's used, and how you do it!
2002 Bioprocess Engineering Pearson College Division
ISBN 0130819085 ISBN-13 9780130819086
This concise yet comprehensive text introduces the essential concepts of bioprocessing - internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information - to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications.
Module Resources
A variety of current topical peer-review papers will be provided via the virtual learning environment (VLE) module page for each lecture to illustrate current thinking on key issues. |
Url Resources |
Links to a variety of current topical peer-review papers will be provided for each lecture to illustrate current thinking on key issues. |
'Bioprocess Engineering - Basic Concepts' by Shuler,M.L. and Kargi F.
Prentice-Hall. 2002. ISBN - 0-13-081908-5.
as above
A variety of current topical peer-review papers will be provided via the virtual learning environment (VLE) module page for each lecture to illustrate current thinking on key issues. |
Url Resources |
Links to a variety of current topical peer-review papers will be provided for each lecture to illustrate current thinking on key issues. |
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