BIO07011 2019 Protein Purification L7.
Full Title
Protein Purification L7.
Transcript Title
Protein Purification L7.
Code
BIO07011
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
LIFE - Life Sciences
Level
07 - Level 7
Credit
10 - 10 Credits
Duration
Semester
Fee
€
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Paul Walsh
Programme Membership
SG_SBIOP_J07 201900 Bachelor of Science in Biopharmaceutical Science
SG_SBIOP_E07 201900 Certificate in Biopharmaceutical Processing
Description
This course aims to provide students with a broad understanding and practical appreciation of the main theoretical concepts and principles of protein purification (downstream processing). This will include both theory some relevant laboratory practicals.
Learning Outcomes
On completion of this module the learner will/should be able to;
1.
Outline the main elements of a downstream process design for protein capture and purification.
2.
Describe the general theory and main principles of chromatography technology for protein purification applications.
3.
Describe the general theroy and main principles of filtration technology for protein purification applications.
4.
Describe the various viral exclusion and elimination technologies for downstream processing.
5.
Perform a range of laboratory and pilot plant practicals relating to protein purification.
6.
Communicate scientific information and data in an appropriate format and manner.
Teaching and Learning Strategies
N/A
Module Assessment Strategies
A combination of continuous assessment strategies, some laboratory experiments and an end-of-term exam.
Repeat Assessments
N/A
Indicative Syllabus
· Introduction to Protein Purification.
· The Design and Operation of an effective Protein Capture System.
· The Theory and Principles of Chromatography for Purification.
· The Theory and Principles of Filtration for Purification.
· Practical chromatography and filtration applications for Purification.
· Viral Exclusion Technologies for Protein Purification.
Coursework & Assessment Breakdown
Coursework & Continuous Assessment
50 %
End of Semester / Year Formal Exam
50 %
Coursework Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | Continuous Assessment Short-form exams and lab. practical write-ups. | Coursework Assessment | Multiple Choice/Short Answer Test | 10 % | Week 5 | 1,2,3,4,5,6 |
| 2 | Assessment by MCQ Online | Coursework Assessment | Multiple Choice/Short Answer Test | 10 % | Week 9 | 1,2,3,4,5,6 |
| 3 | Report Format | Coursework Assessment | Written Report/Essay | 30 % | End of Semester | 1,2,3,4,5,6 |
End of Semester / Year Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | Final Exam | Final Exam | Closed Book Exam | 50 % | End of Term | 1,2,3,4 |
Part Time Mode Workload
| Type | Location | Description | Hours | Frequency | Avg Workload |
|---|---|---|---|---|---|
| Lecture | Distance Learning Suite | Online lecture. | 1 | Weekly | 1.00 |
| Practical / Laboratory | Science Laboratory | Laboratory practical experiments. | 16 | Once Per Semester | 1.07 |
| Directed Learning | Not Specified | Directed self-study. | 5 | Weekly | 5.00 |
Total Part Time Average Weekly Learner Contact Time 2.07 Hours
Module Resources
Non ISBN Literary Resources
‘Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.
Wiley Publications. 2007. ISBN - 978-0-470-01244-4.
'Bioseparations Science and Engineering' by R.G. Harrison, P.Todd, S.R. Rudge, D.P. Petrides. Oxford University Press. 2003. ISBN - 0-19-512340-9.
'Isolation and Purification of Proteins' by R. Hatti-Kaul, B.Mattiasson.
Marcel-Dekker. 2003. ISBN- 0-8247-0726-5.
Journal Resources
N/A
URL Resources
N/A
Other Resources
None
Additional Information
None