BIO06012 2019 Downstream Bioprocessing

General Details

Full Title
Downstream Bioprocessing
Transcript Title
Downstream Bioprocessing
Code
BIO06012
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
LIFE - Life Sciences
Level
06 - Level 6
Credit
10 - 10 Credits
Duration
Semester
Fee
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Stephen Daly, Aodhmar Cadogan
Programme Membership
SG_SBIOP_E06 201900 Certificate in Biopharmaceutical Processing. SG_SBIOP_E06 201900 Certificate in Biopharmaceutical Processing. SG_SGOOD_C06 202300 Higher Certificate in Science in Good Manufacturing Practice and Technology SG_SGOOD_C06 202300 Higher Certificate in Science in Good Manufacturing Practice & Technology
Description

This module aims to provide the student with a fundamental understanding of both the theory and practical aspects of protein purification processing as it pertains to the manufacture of modern biopharmaceutical products.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Describe the principal stages of downstream processing for protein products.

2.

Outline the structure and function of protein biopharmaceutical products.

3.

Outline the main harvesting techniques used for purification of a protein product.

4.

Describe the basic theory and practice of column chromatography for purification applications.  

5.

Describe the basic theory and practice of ultrafiltration and diafiltration operations for protein purification.

6.

Outline the main unit operations required for viral clearance.

7.

 Perform a range of practical laboratory experiments relating to downstream bioprocessing operations.

Teaching and Learning Strategies

This module will be delivered part time.  Is will include lectures, laboratory practicals augmented by independent learning and directed learning.  Moodle will be used as a repository of educational resources.  The laboratory sessions will take place in NIBRT, Dublin.

Module Assessment Strategies

This module is 70% continuous assessment and 30% final exam. 

The continuous assessment is made up of 2 short assessment and practical assessment and reports associated with the practical sessions in NIBRT.

Repeat Assessments

Repeat continuous assessment and/or final exam

Indicative Syllabus

The following is a summary indication of the main topics included in this module:

Protein Structure and Function

Harvesting techniques including centrifugation, microfiltration and depth filtration

Intracellular and extracellular proteins

Filtration and Filter Integrity Testing processes

Ultrafiltration Diafiltration

Chromatography Operations

Viral Clearance

Laboratory practical sessions associated with downstream bioprocessing operations

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
70 %
End of Semester / Year Formal Exam
30 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 MCQ 1 Coursework Assessment Multiple Choice/Short Answer Test 15 % Week 5 1,2,3
2 MCQ 2 Coursework Assessment Multiple Choice/Short Answer Test 15 % Week 11 4,5,6
3 Lab report and assessment Coursework Assessment Assignment 40 % Week 9 7

End of Semester / Year Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Final Exam Final Exam Closed Book Exam 30 % End of Term 1,2,3,4,5,6,7
             
             

Part Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Distance Learning Suite Online lecture. 2 Weekly 2.00
Practical / Laboratory Science Laboratory Lab. practical experiments. 20 Once Per Semester 1.33
Directed Learning Not Specified Directed self-study. 5 Weekly 5.00
Total Part Time Average Weekly Learner Contact Time 3.33 Hours

Module Resources

Non ISBN Literary Resources

‘Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.

Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988

Amersham Pharmacia (GE Healthcare)., Series of Chromatography Manuals for industrial applications.

Journal Resources

None

URL Resources

None

Other Resources

None

Additional Information

None