BIO06012 2019 Downstream Bioprocessing
Full Title
Downstream Bioprocessing
Transcript Title
Downstream Bioprocessing
Code
BIO06012
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
LIFE - Life Sciences
Level
06 - NFQ Level 6
Credit
10 - 10 Credits
Duration
Semester
Fee
€
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Stephen Daly, Aodhmar Cadogan
Programme Membership
SG_SBIOP_E06 201900 Certificate in Biopharmaceutical Processing.
SG_SBIOP_E06 201900 Certificate in Biopharmaceutical Processing.
SG_SGOOD_C06 202300 Higher Certificate in Science in Good Manufacturing Practice and Technology
SG_SGOOD_C06 202300 Higher Certificate in Science in Good Manufacturing Practice & Technology
Description
This module aims to provide the student with a fundamental understanding of both the theory and practical aspects of protein purification processing as it pertains to the manufacture of modern biopharmaceutical products.
Learning Outcomes
On completion of this module the learner will/should be able to;
1.
Describe the principal stages of downstream processing for protein products.
2.
Outline the structure and function of protein biopharmaceutical products.
3.
Outline the main harvesting techniques used for purification of a protein product.
4.
Describe the basic theory and practice of column chromatography for purification applications.
5.
Describe the basic theory and practice of ultrafiltration and diafiltration operations for protein purification.
6.
Outline the main unit operations required for viral clearance.
7.
Perform a range of practical laboratory experiments relating to downstream bioprocessing operations.
Teaching and Learning Strategies
This module will be delivered part time. Is will include lectures, laboratory practicals augmented by independent learning and directed learning. Moodle will be used as a repository of educational resources. The laboratory sessions will take place in NIBRT, Dublin.
Module Assessment Strategies
This module is 70% continuous assessment and 30% final exam.
The continuous assessment is made up of 2 short assessment and practical assessment and reports associated with the practical sessions in NIBRT.
Repeat Assessments
Repeat continuous assessment and/or final exam
Module Dependencies
Prerequisites
None
Co-requisites
None
Incompatibles
None
Indicative Syllabus
The following is a summary indication of the main topics included in this module:
Protein Structure and Function
Harvesting techniques including centrifugation, microfiltration and depth filtration
Intracellular and extracellular proteins
Filtration and Filter Integrity Testing processes
Ultrafiltration Diafiltration
Chromatography Operations
Viral Clearance
Laboratory practical sessions associated with downstream bioprocessing operations
Coursework & Assessment Breakdown
Coursework & Continuous Assessment
70 %
End of Semester / Year Formal Exam
30 %
Coursework Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | MCQ 1 | Coursework Assessment | Multiple Choice/Short Answer Test | 15 % | Week 5 | 1,2,3 |
| 2 | MCQ 2 | Coursework Assessment | Multiple Choice/Short Answer Test | 15 % | Week 11 | 4,5,6 |
| 3 | Lab report and assessment | Coursework Assessment | Assignment | 40 % | Week 9 | 7 |
End of Semester / Year Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | Final Exam | Final Exam | Closed Book Exam | 30 % | End of Term | 1,2,3,4,5,6,7 |
Part Time Mode Workload
| Type | Location | Description | Hours | Frequency | Avg Workload |
|---|---|---|---|---|---|
| Lecture | Distance Learning Suite | Online lecture. | 2 | Weekly | 2.00 |
| Practical / Laboratory | Science Laboratory | Lab. practical experiments. | 20 | Once Per Semester | 1.33 |
| Directed Learning | Not Specified | Directed self-study. | 5 | Weekly | 5.00 |
Total Part Time Average Weekly Learner Contact Time 8.33 Hours
Module Resources
Non ISBN Literary Resources
‘Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.
Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988
Amersham Pharmacia (GE Healthcare)., Series of Chromatography Manuals for industrial applications.
Journal Resources
None
URL Resources
None
Other Resources
None
Additional Information
None